Protein

— High Quality Drug Target Proteins

Insect Baculovirus Expression System

Insect baculovirus expression is a recombinant protein production system that uses recombinant baculoviruses carrying exogenous target genes as vectors for expression in insects. The recombinant proteins can be fully functional as they undergo post-translational modifications such as glycosylation, phosphorylation, acylation, and signal peptide cleavage. These modifications are similar to those in mammalian cells. By using strong promoters like p10 and pH, it can reach a high expression level, can accommodate large-size insertion fragments, and express multiple genes simultaneously. The Biortus team has over a decade of rich experience in protein expression, and has successfully completed more than 9000 batches of protein expression services in total.

Service Details

• Baculovirus preparation (Bac to Bac system、BacMam system);

• Virus titer determination;

• Expression optimization in Sf9, Sf21, and High-5 cells (small-scale expression and purification, FSEC);

• Large-scale expression in insect cell (up to 1200L/month);

Experimental Procedures

  • bacmid preparation 3 days

  • virus amplification 10 days

  • virus titer determination 2 days

  • 10 ml small-scale expression test 3-4 days

  • scale-up expression 2-3 days

Instruments

  • Shaker

  • 15L Fermentor

  • Cell Flowtometry

  • WAVE

Protein

Protein expression