Protease Inhibitor
The Biortus protease inhibitor is a mixture containing multiple protease inhibitors of various types, covering a wide range of protease targets. This product effectively protects the "protease-sensitive" properties of proteins, thereby preventing unwanted proteolytic cleavage that may occur during experiments or biochemical processes.
The components of the protease inhibitor mixture include
| Serine protease inhibitors | Such as AEBSF, Aprotinin, etc., used to inhibit serine protease activity. |
| Cysteine protease inhibitors | Such as E-64, lepeptin, used to inhibit cysteine protease activity. |
| Metalloprotease inhibitors | Such as EDTA (added), used to chelate metal ions required for metalloprotease activity. |
| Aspartic protease inhibitors | Such as Pepstatin A, used to inhibit aspartic protease activity. |
The Biortus protease inhibitor can be used in various forms and is widely applied in experiments such as protein purification, protein immunoprecipitation, cell lysate preparation, and Western-blot experiments to protect target proteins from proteolytic degradation.
The working concentrations of each component of Biortus protease inhibitors
| Component | Mechanism of action | Working concentration |
|---|---|---|
|
AEBSF
(MW: 239.69) |
Serine protease
(irreversible) |
1 mM |
|
Aprotinin
(MW: 6511.44) |
Serine protease
(irreversible) |
0.01~0.3 μM |
|
Bestatin
(MW: 308.37) |
Aminopeptidase
(reversible) |
130 μM |
|
E64
(MW: 357.41) |
Cysteine protease
(irreversible) |
1.4~2.8 μM |
|
Leupeptin
(MW: 475.59) |
Serine and cysteine proteases
(reversible) |
0.03 μM |
|
Pepstatin A
(MW: 685.89) |
Aspartic protease
(reversible) |
1 μM |
Related Products
Biortus provides researchers with high-purity, high-activity TEV protease, HRV3C protease, and SUMO protease.
Experimental Tools 
