HTS
This is the general workflow for fragment-based lead discovery (FBLD) at
Biortus. It outlines the key stages involved in identifying and validating
fragment hits, starting with reagent generation and assay development, followed
by library screening using various assay formats. The process also includes
hits validation with different orthogonal assays and structure confirmation
through co-crystallization or cryo-EM. This demonstrates the "One-Stop" "Gene
to Structure" service that Biortus offers to effectively support your drug
discovery programs.
General Workflow for HTS at Biortus
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Reagent production
Protein generation
Stable cell line generation
Reagent labeling
Assay development & validation
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Screening
Primary screening
Confirmation in CRC
Orthogonal assays
~1-2 weeks
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Structure Confirmation
Co-Crystallization
~4-8 weeks
Covalent compound library screening workflow
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BFL-4: ~1200 covalentfragments
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Echo 650
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Dragonfly
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Compound-protein reaction
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Agilent 6230B TOF
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Masshunter BioConfirm 10.0 software
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Mass_Auto_Gooey software
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HTS Covalent compound screen report
Libraries
